Gilks CF, Brindle RJ, Mwachari C, Batchelor B, Bwayo J, et al. Nontuberculous mycobacteria: defining disease in a prospective cohort of South African miners.
1999 159:94–99.Ĭorbett EL, Blumberg L, Churchyard GJ, Moloi N, Mallory K, et al. Risk factors for pulmonary mycobacterial disease in South African gold miners. Routine molecular confirmation of positive Lowenstein-Jensen cultures is unnecessary in this low resource setting.Ĭorbett EL, Churchyard GJ, Clayton T, Herselman P, Williams B, et al. Presumptive diagnosis of tuberculosis on the basis of a positive Lowenstein-Jensen culture is sufficient in HIV-infected Ugandans suspected of having tuberculosis. (n = 1) and six cultures had organisms that could not be identified. Subsequent analysis of the cultures from 54 patients by PCR and sequence analyses to identify co-infection with NTM confirmed the presence of MTBC as well as the presence of Micrococcus luteus (n = 4), Janibacter spp.
Sputum and bronchoalveolar lavage Lowenstein-Jensen mycobacterial culture isolates from consecutive, HIV-infected patients admitted to Mulago Hospital with 2 weeks or more of cough were subjected to IS6110 PCR and rpoB genetic analysis to determine the presence of Mycobacterium tuberculosis complex (MTBC) and non-tuberculous mycobacteria (NTM).Įighty (100%) mycobacterial cultures from 65 patients were confirmed to be members of MTBC. To determine the need for routine speciation of positive Lowenstein-Jensen mycobacterial cultures in HIV-infected patients suspected of having pulmonary tuberculosis at Mulago Hospital in Kampala, Uganda.
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